Overview

  • Product nameAnti-Kallikrein 5 antibody
    See all Kallikrein 5 primary antibodies
  • Description
    Rabbit polyclonal to Kallikrein 5
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 280-293 of Human KLK5.

  • Positive control
    • MCF-7 and ZR75-1 cell lysates (see review)

Properties

Applications

Our Abpromise guarantee covers the use of ab7283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionMay be involved in desquamation.
  • Tissue specificityExpressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HK5 antibody
    • Kallikrein 5 antibody
    • Kallikrein L2 antibody
    • Kallikrein like 2 antibody
    • Kallikrein like protein 2 antibody
    • Kallikrein related peptidase 5 antibody
    • Kallikrein-5 antibody
    • Kallikrein-like protein 2 antibody
    • Kallikrein5 antibody
    • KLK 5 antibody
    • KLK L2 antibody
    • KLK-L2 antibody
    • KLK5 antibody
    • KLK5_HUMAN antibody
    • KLKL 2 antibody
    • KLKL2 antibody
    • SCTE antibody
    • Stratum corneum tryptic enzyme antibody
    • UNQ570/PRO1132 antibody
    see all

Anti-Kallikrein 5 antibody images

  • IHC image of ab7283 staining Kallikrein 5 in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab7283 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7283, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Kallikrein 5 antibody (ab7283)

This product has been referenced in:
  • Furio L  et al. Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of Netherton syndrome. J Exp Med 211:499-513 (2014). Read more (PubMed: 24534191) »
  • Bonnart C  et al. Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing. J Clin Invest 120:871-82 (2010). IHC-P ; Human . Read more (PubMed: 20179351) »

See all 4 Publications for this product

Product Wall

L’immunogène d’ab7283 correspond aux acides amines 280-293 de la protéine kallikrein 5 humaine. Ces acides aminés sont présents chez la forme active de la protéine.

L’immunogène d&rsq...

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Please find attached the Material Safety Datasheet for some of the products requested. Those products not containing any hazardous products do not require MSDS.
If by any chance you required these datasheets for l...

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Thank you for your enquiry. The antibody ab7283 has been tested in standard reducing conditions in western blotting and has not been tested in IHC-P. It may work in IHC-P and should you decide to go ahead and purchase this product, please let us know h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"