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Western blot - Kallikrein 5 antibody - Kallikrein loop (ab28565)
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Product Name
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Kallikrein 5 antibody - Kallikrein loop
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See all Kallikrein 5 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Kallikrein 5 - Kallikrein loop
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Immunogen
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Synthetic peptide based on the Kallikrein loop area of human Kallikrein 5.
(Peptide available as ab41423.)
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with Kallikrein 5, but does not react with the other Kallikreins (1-7 and 9-15).
We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 5 antibodies
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT)- 1/5000 (chemiluminescent substrates). Predicted molecular weight: 32 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Relevance
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Kallikrein 5 was originally described from the skin as an enzyme involved in turnover of the stratum corneum, and later cloned from human EST libraries as a Kallikrein like enzyme. The skin is known to contain trypsin like and chymotrypsin like proteinases of 32 kDa and 25 kDa respectively, and these were determined to be hK5 and hK7. Kallikrein 5 is found is greatest abundance in the skin, and also in the brain, with lesser signal in the kidney, lung, mammary gland, and testis. Human breast milk is reported to contain high levels of Kallikrein 5. Less stringent PCR detects hK5 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK5.
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Kallikrein 5 antibody - Kallikrein loop (ab28565) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Kallikrein 5 antibody - Kallikrein loop is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Kallikrein 5 antibody - Kallikrein loop images, references, products related to ab28565 and other tools.
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Kallikrein 5 antibody - Kallikrein loop images:
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 Western blot - Kallikrein 5 antibody - Kallikrein loop (ab28565)
All lanes : Kallikrein 5 antibody - Kallikrein loop (ab28565) at 1/1000 dilution
Lane 1 : Kallikrein 5 at 0.05 µg
Lane 2 : Kallikrein 5 at 0.01 µg
Lane 3 : Kallikrein 5 at 0.001 µg
Predicted band size : 32 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Kallikrein 5
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Kallikrein 5 antibody - Kallikrein loop - more information
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Customer reviews (feedback) regarding Kallikrein 5 antibody - Kallikrein loop |
Customer FAQs regarding Kallikrein 5 antibody - Kallikrein loop |
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