Anti-Kallikrein 5 antibody - Kallikrein loop (ab28565)

Overview

  • Product name
    Anti-Kallikrein 5 antibody - Kallikrein loop
    See all Kallikrein 5 primary antibodies
  • Description
    Rabbit polyclonal to Kallikrein 5 - Kallikrein loop
  • Specificity
    This antibody reacts with Kallikrein 5, but does not react with the other Kallikreins (1-7 and 9-15).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the Kallikrein loop area of human Kallikrein 5.

    (Peptide available as ab41423.)

Properties

Applications

Our Abpromise guarantee covers the use of ab28565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa. 1/1000 (when using colorimetric substrates such as BCIP/NBT)- 1/5000 (chemiluminescent substrates). Dilution optimised using Chromogenic detection.
ICC/IF Use at an assay dependent dilution. PubMed: 21163944

Target

  • Function
    May be involved in desquamation.
  • Tissue specificity
    Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HK5 antibody
    • Kallikrein 5 antibody
    • Kallikrein L2 antibody
    • Kallikrein like 2 antibody
    • Kallikrein like protein 2 antibody
    • Kallikrein related peptidase 5 antibody
    • Kallikrein-5 antibody
    • Kallikrein-like protein 2 antibody
    • Kallikrein5 antibody
    • KLK 5 antibody
    • KLK L2 antibody
    • KLK-L2 antibody
    • KLK5 antibody
    • KLK5_HUMAN antibody
    • KLKL 2 antibody
    • KLKL2 antibody
    • SCTE antibody
    • Stratum corneum tryptic enzyme antibody
    • UNQ570/PRO1132 antibody
    see all

Images

  • All lanes : Anti-Kallikrein 5 antibody - Kallikrein loop (ab28565) at 1/1000 dilution

    Lane 1 : Kallikrein 5 at 0.05 µg
    Lane 2 : Kallikrein 5 at 0.01 µg
    Lane 3 : Kallikrein 5 at 0.001 µg


    Predicted band size : 32 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Jiang R  et al. Kallikrein-5 promotes cleavage of desmoglein-1 and loss of cell-cell cohesion in oral squamous cell carcinoma. J Biol Chem 286:9127-35 (2011). ICC/IF ; Human . Read more (PubMed: 21163944) »
  • Ghosh S  et al. Urinary-type plasminogen activator receptor/alpha 3 beta 1 integrin signaling, altered gene expression, and oral tumor progression. Mol Cancer Res 8:145-58 (2010). IHC-P ; Mouse . Read more (PubMed: 20145038) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Trititon X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

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Verified customer

Submitted Aug 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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