Kallikrein 6 antibody - Catalytic domain (ab40952)

Product Name 

Kallikrein 6 antibody - Catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Kallikrein 6 - Catalytic domain

Immunogen 

Synthetic peptide based on the catalytic domain of Human Kallikrein 6.

(Peptide available as ab43088.)

Reacts with 
(species key)

Hu

Specificity 

Ab40952 reacts with human Kallikrein 6 and does not react with the other human kallikreins (hK1-5, 7-15).

We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 6 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 26.9 kDa. The processed hK6 has an apparent mass of 31-33 kDa on reduced SDS PAGE gels. A range of different sized bands can be seen for Kallikrein-6 in different tissues and in cell culture media, ranging from 16-32 kDa, probably representing different proteolytically processed forms but perhaps also the different splice variants. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Neuroscience >> Neurodegeneration >> Alzheimer's >> Other

Relevance 

Kallikreins (KLKs) are a subgroup of trypsin-like serine proteases and are implicated in carcinogenesis. Originally described from human urine as a hypotensive agent and later from the pancreas, kallkreins were given the Greek name for pancreas, kallikreas.

Kallikrein 6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein 6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP) and hK6 was thought to be a beta secretase. Kallikrein 6 was shown to be elevated in the sera patients with Alzheimer’s disease and Parkinson’s disease and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin. Kallikrein 6 is found in greatest abundance in the brain and neuronal tissues, with lesser levels in the kidney, thymus, spleen and lymph nodes. Human breast milk is reported to contain high levels of Kallikrein 6 and hK6 has been purified from cerebral spinal fluid (CSF). Less stringent PCR detects hK6 in many tissues and analysis of cell lines by Western blot shows that many cell lines make hK6. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 6 can be found complexed to a number of different proteinase inhibitors.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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