Kallikrein 7 antibody - Catalytic domain (ab40953)

Product Name 

Kallikrein 7 antibody - Catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Kallikrein 7 - Catalytic domain

Immunogen 

Synthetic peptide based on the catalytic domain of Human Kallikrein 7.

(Peptide available as ab43089.)

Reacts with 
(species key)

Hu

Specificity 

Ab40953 reacts with human Kallikrein 7 and does not react with the other human kallikreins (hK1-6, 8-15).

We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 7 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 27.6 kDa. A shorter sequence starting at the second methionine has been reported. The 181 amino acid protein has a predicted mass of 19.9 kDa. This shorter sequence, if produced as a protein, would lack the cysteine of the catalytic triad and is thus, likely to be proteolytically inactive. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins

Relevance 

The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp, and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer.

Kallikrein 7, also known as stratum corneum chymotryptic enzyme (SCCE) and PRSS6, is a chymotrypsin-like serine proteinase. Originally described from human skin as a serine protease involved in shedding of skin cells and remodeling if the skin, SCCE was later identified as Kallikrein 7. Kallikrein 7 is found at the highest levels in the skin, often complexed with the endogenous serpins SLPI or elafin and kallikrein 7 can be found complexed to a number of different proteinase inhibitors. In addition to skin, Kallikrein 7 has been found in the kidney, esophagus, neuronal tissues, amniotic fluid, saliva, breast milk, urine, synovial fluid, seminal plasma and serum. Kallikrein 7 has been reported to be decreased in the CSF of Alzheimer's patients and message levels of KLK7 were decreased in adenocarcinoma. In skin, overexpression of hK7 has been shown to cause a form of dermatitis and in psoriasis hK7 is expressed in higher levels than controls. The skin adhesive proteins corneodesmosin and desmocollin 1 have been reported to be substrates of Kallikrein 7, as is interleukin 1 and the insulin B-chain.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for Kallikrein 7 antibody - Catalytic domain images, references, products related to ab40953 and other tools.

Kallikrein 7 antibody - Catalytic domain images:

Customer reviews (feedback) regarding Kallikrein 7 antibody - Catalytic domain

Customer FAQs regarding Kallikrein 7 antibody - Catalytic domain

Protocols for Kallikrein 7 antibody - Catalytic domain

Price and availability of products related to Kallikrein 7 antibody - Catalytic domain

Kallikrein 7 antibody - Catalytic domain - related products: