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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 8 antibody - Catalytic domain
See all Kallikrein 8 products (5) ...
Description
Rabbit polyclonal to Kallikrein 8 - Catalytic domain
Specificity
Ab 40954 reacts with human Kallikrein 8 and does not react with the other human kallikreins (hK1-7, 9-15).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the catalytic domain of Human Kallikrein 8.
(Peptide available as ab43090.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 8 antibody - Catalytic domain (ab40954)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 33 kDa. A number of different splice variants and partial sequences have been reported for Kallikrein 8, the longest encodes a protein of 305 amino acids with a predicted mass of 32.97 kDa. The 260 amino acid form has a predicted mass of 28 kDa. The 164, 134 and 119 amino acid versions have a predicted mass of 17.9, 14.7 and 12.7 kDa respectively but it is unclear if these proteins are produced. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.
Tissue specificity
Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted. Cytoplasm. Shows a cytoplasmic distribution in the keratinocytes.
Target information above from: UniProt accessionO60259
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ovasin antibody
- Serine protease TADG 14 antibody
- Tumor associated differentially expressed gene 14 protein antibody
see all
Database links
- Entrez Gene: 11202 Human
- Omim: 605644 Human
- SwissProt: O60259 Human
- Unigene: 104570 Human
Anti-Kallikrein 8 antibody - Catalytic domain images:
Western blot - Kallikrein 8 antibody - Catalytic domain (ab40954)
All lanes : Anti-Kallikrein 8 antibody - Catalytic domain (ab40954) at 1/1000 dilution
Lane 1 : Kallikrein 8 at 0.05 µg
Lane 2 : Kallikrein 8 at 0.01 µg
Lane 3 : Kallikrein 8 at 0.001 µg
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 66 kDa (possible dimer).
Protocols
References for Anti-Kallikrein 8 antibody - Catalytic domain (ab40954)
ab40954 has not yet been referenced specifically in any publications.
Publishing research using ab40954? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"