Overview

  • Product nameAnti-Kallikrein 9 antibody
    See all Kallikrein 9 primary antibodies
  • Description
    Rabbit polyclonal to Kallikrein 9
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 239-250 of Human KLK9.

  • General notes


    This antibody recognizes the gene product hk9 of human KLK9 (also known as KLK-L3), one of the many kallikrein serine proteases associated with carcinogenesis. KLK9 is expressed in thymus, testis, spinal cord, salivary gland, ovary and skin. Like other kallikreins, KLK9 is regulated by steroid hormones in cancer cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab7284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Tissue specificitySkin, thymus, trachea, cerebellum and spinal cord.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kallikrein 8 antibody
    • Kallikrein 9 antibody
    • Kallikrein L3 antibody
    • Kallikrein Like 3 antibody
    • Kallikrein like protein 3 antibody
    • kallikrein related peptidase 9 antibody
    • Kallikrein-9 antibody
    • Kallikrein-like protein 3 antibody
    • Kallikrein8 antibody
    • Kallikrein9 antibody
    • KLK 8 antibody
    • KLK 9 antibody
    • KLK L3 antibody
    • KLK-L3 antibody
    • KLK8 antibody
    • KLK9 antibody
    • KLK9_HUMAN antibody
    • KLKL 3 antibody
    • KLKL3 antibody
    see all

Anti-Kallikrein 9 antibody images

  • ICC/IF image of ab7284 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Kallikrein 9 antibody (ab7284)

ab7284 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"