Overview

  • Product name
  • Description
    Rabbit polyclonal to KANK4
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 377-526 of Human KANK4.

  • Positive control
    • Human colon tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Contains 5 ANK repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANKRD38 antibody
    • Ankyrin repeat domain 38 antibody
    • Ankyrin repeat domain-containing protein 38 antibody
    • dJ1078M7.1 antibody
    • Kank4 antibody
    • KANK4_HUMAN antibody
    • Kidney ankyrin repeat containing protein 4 antibody
    • KN motif and ankyrin repeat domain-containing protein 4 antibody
    • KN motif and ankyrin repeat domains 4 antibody
    • RP5-1155K23.5 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab121410 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121410 at 1/50 dilution staining KANK4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-KANK4 antibody (ab121410) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

References

This product has been referenced in:
  • Afshari NA  et al. Genome-wide association study identifies three novel loci in Fuchs endothelial corneal dystrophy. Nat Commun 8:14898 (2017). Read more (PubMed: 28358029) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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