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A synthetic phosphorylated peptide, corresponding to a sequence of human KAP1 surrounding Serine 824. NP_005753.1
Our Abpromise guarantee covers the use of ab70369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 89 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
Detection of Human KAP1 (phospho S824) by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells that were mock treated (-) or treated with Etoposide (100 µM, 2h).
Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of KAP1 (phospho S824) by ab70369 (1:200) in NBF-fixed HeLa cells by Immunocytochemistry.
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