Anti-Kappa free light chains antibody [4C11] (ab1939)

Overview

  • Product nameAnti-Kappa free light chains antibody [4C11]
    See all Kappa free light chains primary antibodies
  • Description
    Mouse monoclonal [4C11] to Kappa free light chains
  • SpecificitySpecific for  independent constantly expressed epitope of kappa chain. Reacts with free monomer and dimer of  kappa light chains but not with intact kappa bearing immunoglobulins.
  • Tested applicationsSuitable for: ELISA, WB, Flow Cyt, Othermore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of light chains of human immunoglobulin.

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone number4C11
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Specific antibody titer is 1/200 000 by indirect ELISA
AP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
Flow Cyt Use 2µg for 106 cells.
Other Use at an assay dependent concentration.

Target

  • Database links
  • Alternative names
    • Free kappa light chain antibody
    • Free kappa light chains antibody
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • Kappa free light chain antibody
    • Kappa light chain free antibody
    • Kappa light chains free antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Kappa free light chains antibody [4C11] images

  • Overlay histogram showing Ramos cells stained with ab1939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1939, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Kappa free light chains antibody [4C11] (ab1939)

ab1939 has not yet been referenced specifically in any publications.

Product Wall

We only have information that ab1939 Kappa free light chains antibody [4C11] is specific for an independent constantly expressed epitope of kappa chain and that it reacts with free monomer and dimer of kappa light chains but not with intact kappa beari...

Read More

I am sorry for the delay in getting back to you.

I now have further information to share with you in regards to the samples used with the Anti-Kappa free light chains antibody [4C11] (ab1939). As we have discussed previously, this antibody ha...

Read More

Thank you for contacting us today.

I am sorry for the delay in getting back to you. As discussed over the phone, as you intend to use the antibodies ab1939, ab109489 and ab134083 in Western blotting and IHC, we would be able to offer you a te...

Read More

Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

The protocolused for Western blotting was according to Towbin et al. in: PNAS, September 1, 1979, vol. 76, no. 9, 4350-435...

Read More

Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab1939  has never been tested in ICC or IHC-P or IHC-Fr.  All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any ne...

Read More

Thank you for your enquiry. Mab against free light chains of human Ig were selected on the basis of their reactivity with purified Bence-Jones proteins kappa- or lambda and absence of any reaction with whole molecules of IgA, IgG, IgM and IgE. Any...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"