Anti-Kappa free light chains antibody [5H11] (ab54312)


  • Product nameAnti-Kappa free light chains antibody [5H11]
    See all Kappa free light chains primary antibodies
  • Description
    Mouse monoclonal [5H11] to Kappa free light chains
  • Tested applicationsSuitable for: ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of light chains of human immunoglobulin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number5H11
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab54312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • Database links
  • Alternative names
    • Free kappa light chain antibody
    • Free kappa light chains antibody
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • Kappa free light chain antibody
    • Kappa light chain free antibody
    • Kappa light chains free antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Kappa free light chains antibody [5H11] images

  • Overlay histogram showing Ramos cells stained with ab54312 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54312, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Kappa free light chains antibody [5H11] (ab54312)

ab54312 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood cells)
Permeabilization No
Gating Strategy Lymphoid gate only
Specification blood cells
Preparation Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin (PBS-GEL)
Fixation None

Dr. Marek Sinkora

Verified customer

Submitted Jun 21 2016