Anti-Kappa light chain antibody (Alkaline Phosphatase) (ab49744)


  • Product nameAnti-Kappa light chain antibody (Alkaline Phosphatase)
    See all Kappa light chain primary antibodies
  • Description
    Goat polyclonal to Kappa light chain (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • SpecificityReacts with both bound and free Human kappa light chain.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Dot Blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Bence Jones kappa light chain

  • Positive control
    • Human tonsil
  • General notes

    Goat anti-human kappa light chain is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 50% Glycerol, 1% BSA, 0.05M Tris buffered saline, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • Purification notesIsolated by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the human kappa light chain.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab49744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/30000. Predicted molecular weight: 12 kDa.
ELISA 1/30000.
IHC-P 1/50.
Dot Blot 1/30000.


  • RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
  • Alternative names
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

References for Anti-Kappa light chain antibody (Alkaline Phosphatase) (ab49744)

ab49744 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (bevacizumab)
Loading amount 0.025 µg
Specification bevacizumab
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Lukasz Kacprzyk

Verified customer

Submitted Mar 12 2013

Thank you for contacting us.

You are correct!. ab49744 react with kappa light and ab49365 with heavy chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.