Recombinant
RabMAb

Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Overview

  • Product name
    Anti-Kappa light chain antibody [EPR5367-8]
    See all Kappa light chain primary antibodies
  • Description
    Rabbit monoclonal [EPR5367-8] to Kappa light chain
  • Tested applications
    Suitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Human IgA.

  • Positive control
    • WB: Human tonsil, plasma and spleen tissue lysates. IHC-P: Human tonsil tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5367-8
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
IP 1/10 - 1/100.
ELISA 1/16000.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • All lanes : Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

    Lane 1 : Human tonsil cell lysate
    Lane 2 : Human plasma cell lysate
    Lane 3 : Human spleen cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 25 kDa
  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab124727 at 1/250-500 dilution

References

ab124727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Merci de nous avoir contactés.

Nous avons un anticorps semblable à l'anticorps A7164. C'est un monoclonal de lapin anti-Human IgG Kappa Light Chain, clone[EPR5367], référence ab124727, http://www.abcam.com/ab124727....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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