Anti-Kappa light chain antibody (FITC) (ab4208)

Overview

  • Product nameAnti-Kappa light chain antibody (FITC)
    See all Kappa light chain primary antibodies
  • Description
    Rabbit polyclonal to Kappa light chain (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Pool of human kappa light chain isolated from the urine of patients with Bence Jones Proteinuria.

  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesTraces of contaminating antibodies have been removed by solid phase absorption. Only the kappa precipitation line appeared in cross immunoelectrophoresis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/40. Incubation for 30-60 minutes at room temperature is suggested.
IHC-Fr Use at an assay dependent dilution.

Target

  • RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
  • Alternative names
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

References for Anti-Kappa light chain antibody (FITC) (ab4208)

ab4208 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I can confirm that the buffer is PBS with BSA and sod. azide. No Triton-X was included.

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