Anti-Kappa light chain antibody (HRP) (ab46527)

Overview

  • Product nameAnti-Kappa light chain antibody (HRP)
    See all Kappa light chain primary antibodies
  • Description
    Goat polyclonal to Kappa light chain (HRP)
  • ConjugationHRP
  • SpecificityThis antibody recognises mouse kappa light chains; both bound and free.
  • Tested applicationsSuitable for: IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse paraproteins with kappa light chains

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody was made from pooled antisera from goats immunised with mouse paraproteins bearing kappa light chains. Antiserum was purified by affinity chromatography on pooled mouse Igs with kappa light chains. The antiserum was cross adsorbed on pooled mouse Igs with lambda light chains.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 12 kDa.

Target

  • RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
  • Alternative names
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

References for Anti-Kappa light chain antibody (HRP) (ab46527)

ab46527 has not yet been referenced specifically in any publications.

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