- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-Kappa light chain antibody [KP53], prediluted
See all Kappa light chain primary antibodies - DescriptionMouse monoclonal [KP53] to Kappa light chain, prediluted
- SpecificityThis antibody reacts with B cell follicles in human lymphoid tissues. The mantle zones give a mosaic pattern, while the germinal centers show a coarse meshwork pattern of staining. This antibody stains free as well as bound kappa light chains. This antibody does not react with human lambda light chain.
- Tested applicationsSuitable for: IHC-P, IHC-Frmore details
- Species reactivityReacts with: Human
Properties
- FormPrediluted
- Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: Inert stabilizer -
Concentration information loading... - ClonalityMonoclonal
- Clone numberKP53
- Myelomaunknown
- IsotypeIgG2b
- Light chain typeunknown
- Research areas
Applications
Our Abpromise guarantee covers the use of ab866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | ||
| IHC-Fr |
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Athough not required, enzymatic predigestion may help in some cases.
Target
- RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
- Cellular localizationCytoplasmic and Secreted
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Database links
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01834 Human
- Unigene: 449609 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Anti-Kappa light chain antibody [KP53], prediluted images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [KP53], prediluted (ab866)](http://a.abcam.com/ps/products/0/ab866/Images/ab866-1-D004.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [KP53], prediluted (ab866)Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections, labelling Kappa Light Chain with ab866.
References for Anti-Kappa light chain antibody [KP53], prediluted (ab866)
ab866 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"