Anti-Kappa light chain antibody [L1C1] (Biotin) (ab79033)

Overview

  • Product nameAnti-Kappa light chain antibody [L1C1] (Biotin)
    See all Kappa light chain primary antibodies
  • Description
    Mouse monoclonal [L1C1] to Kappa light chain (Biotin)
  • ConjugationBiotin
  • SpecificityWe have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    B lymphoma cells

  • Positive control
    • Human tonsil tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberL1C1
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/200 for 20 min at RT using a highsensitivity detection system.
    Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
    • Cellular localizationCytoplasmic and Secreted
    • Database links
    • Alternative names
      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Anti-Kappa light chain antibody [L1C1] (Biotin) images

    • ab79033 at 1/200 dilution staining Kappa light chain in human tonsil by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of B lymphocytes. The purified form of this antibody was used for staining.

    References for Anti-Kappa light chain antibody [L1C1] (Biotin) (ab79033)

    ab79033 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"