Anti-Kappa light chain antibody [L1C1] (FITC) (ab1185)

Overview

  • Product name
    Anti-Kappa light chain antibody [L1C1] (FITC)
    See all Kappa light chain primary antibodies
  • Description
    Mouse monoclonal [L1C1] to Kappa light chain (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with kappa light chains of human immunoglobulins.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • General notes

    This antigen is present in serum on all immunoglobulins bearing kappa light chains. It is also present on approximately 50% of normal human B lymphocytes.

    Measurement of kappa light chain in human lysed whole peripheral blood or mononuclear cells separated by density gradient. Studies of clonality of B cell lymphomas in tissue. Studies of clonality of B cell leukemias in peripheral blood. Kappa (FITC) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl of Kappa (FITC) is sufficient for labelling of 1x106 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    PBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • Primary antibody notes
    This antigen is present in serum on all immunoglobulins bearing kappa light chains. It is also present on approximately 50% of normal human B lymphocytes.
  • Clonality
    Monoclonal
  • Clone number
    L1C1
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

References for Anti-Kappa light chain antibody [L1C1] (FITC) (ab1185)

ab1185 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

For product ab1185 we send 500uL, and for product ab91346 the volume is 2ml.

There aren’t significant differences between antibodies. Both are quite similar, but because of business related issues,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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