Anti-Kappa light chain antibody [MEM-09] (ab1050)


  • Product nameAnti-Kappa light chain antibody [MEM-09]
    See all Kappa light chain primary antibodies
  • Description
    Mouse monoclonal [MEM-09] to Kappa light chain
  • SpecificityMAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM.
  • Tested applicationsSuitable for: IP, WB, IHC-P, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). (Crude thymus membrane fraction).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by protein A-affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberMEM-09
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab1050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 µg/ml.
ICC Use at an assay dependent concentration.


  • RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
  • Alternative names
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Kappa light chain antibody [MEM-09] images

  • Anti-Kappa light chain antibody [MEM-09] (ab1050) at 1/2000 dilution + Human B lymphocytes, whole cell lysate at 15 µg

    Alkaline phosphatase conjugated goat anti-mouse antibody at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 11 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Kappa light chain antibody [MEM-09] (ab1050)

This product has been referenced in:
  • O'Callaghan PM  et al. Cell line-specific control of recombinant monoclonal antibody production by CHO cells. Biotechnol Bioeng 106:938-51 (2010). WB ; Chinese Hamster . Read more (PubMed: 20589672) »
  • Horejsi­ V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). Read more (PubMed: 2422063) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (B lymphocytes)
Loading amount 15 µg
Specification B lymphocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 06 2008

Since the immunoglobulin concentration of this product is 1mg/ml, I would suggest a starting concentration of 1ug/ml. Adjust this accordingly from the results obtained.