Anti-Kappa Opioid Receptor antibody (ab113533)

Overview

  • Product nameAnti-Kappa Opioid Receptor antibody
    See all Kappa Opioid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Kappa Opioid Receptor
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Kappa Opioid Receptor aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P41145
    (Peptide available as ab138734)

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysates as well as the following Human tissue lysates: Brain; Spinal Cord; Placenta; Small Intestine. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab113533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionInhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • K-OR-1 antibody
    • Kappa opioid receptor antibody
    • Kappa type opioid receptor antibody
    • Kappa-type opioid receptor antibody
    • KOR 1 antibody
    • KOR antibody
    • KOR-1 antibody
    • Opiate receptor kappa 1 antibody
    • Opiate receptor, kappa 1 antibody
    • Opioid receptor, kappa 1 antibody
    • OPRK 1 antibody
    • OPRK antibody
    • OPRK_HUMAN antibody
    • OPRK1 antibody
    • Oprk2 antibody
    • R21 antibody
    see all

Anti-Kappa Opioid Receptor antibody images

  • All lanes : Anti-Kappa Opioid Receptor antibody (ab113533) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 20 kDa,33 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab113533 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113533 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Kappa Opioid Receptor staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113533, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Kappa Opioid Receptor antibody (ab113533)

ab113533 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (spinal cord)
Gel Running Conditions Reduced Denaturing (10%)
Specification spinal cord
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Ms. Louise Faggionato Kimura Vieira

Verified customer

Submitted Jul 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"