The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionInhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Western blot - Kappa Opioid Receptor antibody (ab83293)
Anti-Kappa Opioid Receptor antibody (ab83293) at 1 µg/ml + Fetal brain lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 43 kDa Observed band size : 43 kDa
References for Anti-Kappa Opioid Receptor antibody (ab83293)
This product has been referenced in:
Quelch DR et al. Imaging endogenous opioid peptide release with [(11)C]carfentanil and [(3)H]diprenorphine: influence of agonist-induced internalization. J Cereb Blood Flow MetabN/A:N/A (2014).
Read more (PubMed: 25005876) »