Overview

  • Product name
  • Description
    Rabbit polyclonal to KAT1 / HAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 36-419 of Human KAT1/ HAT1 (Uniprot: O14929)

  • Positive control
    • NT2D1; U87-MG or MCF7 cell lysates, Hela cells, Human colon tissue
  • General notes
    Keep as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
  • Sequence similarities
    Belongs to the HAT1 family.
  • Developmental stage
    Highly expressed in mitotic cells (at protein level).
  • Cellular localization
    Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAT 1 antibody
    • hat1 antibody
    • HAT1_HUMAN antibody
    • Histidine aminotransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase type B catalytic subunit antibody
    • KAT1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human colon tissue, labeling KAT1 / HAT1 with ab155414 at 1/250.
  • Immunofluorescence analysis of HeLa cells, prefixed in 4% paraformaldehyde at room temperature for 15 min, labeling KAT1 / HAT1 with ab155414 at 1/500 (Green). Lower image shows costaining with Hoechst 33342 (Blue).
  • Anti-KAT1 / HAT1 antibody (ab155414) at 1/10000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE

References

ab155414 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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