The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 49 kDa).
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
1/100 - 1/1000.
Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Belongs to the HAT1 family.
Highly expressed in mitotic cells (at protein level).
Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
Immunofluorescence analysis of HeLa cells, prefixed in 4% paraformaldehyde at room temperature for 15 min, labeling KAT1 / HAT1 with ab155414 at 1/500 (Green). Lower image shows costaining with Hoechst 33342 (Blue).
Western blot - Anti-KAT1 / HAT1 antibody (ab155414)
Anti-KAT1 / HAT1 antibody (ab155414) at 1/10000 dilution + U87-MG whole cell lysate at 30 µg