Overview

  • Product nameAnti-KAT1 / HAT1 antibody
    See all KAT1 / HAT1 primary antibodies
  • Description
    Rabbit polyclonal to KAT1 / HAT1
  • Specificityab61225 detects endogenous levels of total HAT protein
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human HAT1.

  • Positive control
    • Extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab61225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionAcetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
  • Sequence similaritiesBelongs to the HAT1 family.
  • Developmental stageHighly expressed in mitotic cells (at protein level).
  • Cellular localizationNucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAT 1 antibody
    • hat1 antibody
    • HAT1_HUMAN antibody
    • Histidine aminotransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase type B catalytic subunit antibody
    • KAT1 antibody
    see all

Anti-KAT1 / HAT1 antibody images

  • All lanes : Anti-KAT1 / HAT1 antibody (ab61225) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa

References for Anti-KAT1 / HAT1 antibody (ab61225)

This product has been referenced in:
  • Zhou X  et al. Hepatic FoxO1 acetylation is involved in oleanolic acid-induced memory of glycemic control: novel findings from Study 2. PLoS One 9:e107231 (2014). WB ; Mouse . Read more (PubMed: 25222566) »
  • Ejlassi-Lassallette A  et al. H4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo. Mol Biol Cell 22:245-55 (2011). WB ; Other . Read more (PubMed: 21118997) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"