The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
FunctionAcetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Sequence similaritiesBelongs to the HAT1 family.
Developmental stageHighly expressed in mitotic cells (at protein level).
Cellular localizationNucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
Histone acetyltransferase type B catalytic subunit antibody
Anti-KAT1 / HAT1 antibody images
Western blot - KAT1 / HAT1 antibody (ab61225)
All lanes : Anti-KAT1 / HAT1 antibody (ab61225) at 1/500 dilution
Lane 1 : extracts from LOVO cells Lane 2 : extracts from LOVO cells with immunizing peptide
Predicted band size : 49 kDa Observed band size : 49 kDa
References for Anti-KAT1 / HAT1 antibody (ab61225)
This product has been referenced in:
Zhou X et al. Hepatic FoxO1 acetylation is involved in oleanolic acid-induced memory of glycemic control: novel findings from Study 2. PLoS One9:e107231 (2014).
Read more (PubMed: 25222566) »
Ejlassi-Lassallette A et al. H4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo. Mol Biol Cell22:245-55 (2011).
Read more (PubMed: 21118997) »