Validated using a knockout cell line
Recombinant
RabMAb

Anti-KAT1 / HAT1 antibody [EPR18661] (ab193097)

Overview

  • Product name
    Anti-KAT1 / HAT1 antibody [EPR18661]
    See all KAT1 / HAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR18661] to KAT1 / HAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KAT1/ HAT1 aa 1-200. The exact sequence is proprietary.
    Database link: O14929

  • Positive control
    • WB: HeLa, MCF7, F9, LLC, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; mouse thymus lysate; human fetal brain, fetal kidney, fetal heart and fetal spleen lysates; mouse brain, heart and kidney lysates; rat brain, heart and kidney lysates. IHC-P: Human tonsil; mouse and rat colon tissues. IP: F9 and HeLa whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab193097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
IP 1/100.

Target

  • Function
    Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
  • Sequence similarities
    Belongs to the HAT1 family.
  • Developmental stage
    Highly expressed in mitotic cells (at protein level).
  • Cellular localization
    Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAT 1 antibody
    • hat1 antibody
    • HAT1_HUMAN antibody
    • Histidine aminotransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase type B catalytic subunit antibody
    • KAT1 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: KAT1 / HAT1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: NIH3T3 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab193097 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab193097 was shown to specifically react with KAT1 / HAT1 when KAT1 / HAT1 knockout samples were used. Wild-type and KAT1 / HAT1 knockout samples were subjected to SDS-PAGE. ab193097 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling KAT1 / HAT1 with ab193097 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human tonsil is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-KAT1 / HAT1 antibody [EPR18661] (ab193097) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : F9 (Mouse embryonic carcinoma cell line) whole cell lysate
    Lane 4 : LLC (Mouse lung carcinoma) whole cell lysate
    Lane 5 : Mouse thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KAT1 / HAT1 with ab193097 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-KAT1 / HAT1 antibody [EPR18661] (ab193097) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KAT1 / HAT1 with ab193097 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-KAT1 / HAT1 antibody [EPR18661] (ab193097)

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KAT1 / HAT1 antibody [EPR18661] (ab193097) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • KAT1 / HAT1 was immunoprecipitated from 1mg of F9 (Mouse embryonic carcinoma cell line) whole cell lysate with ab193097 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193097 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: F9 whole cell lysate 10ug (Input).

    Lane 2: ab193097 IP in F9 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193097 in F9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • KAT1 / HAT1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab193097 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193097 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input).

    Lane 2: ab193097 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193097 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

ab193097 has not yet been referenced specifically in any publications.

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