Anti-KAT13A / SRC1 antibody (ab10308)

Overview

  • Product nameAnti-KAT13A / SRC1 antibody
    See all KAT13A / SRC1 primary antibodies
  • Description
    Rabbit polyclonal to KAT13A / SRC1
  • Tested applicationsSuitable for: IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • Immunogen

    Recombinant fragment corresponding to Human KAT13A/ SRC1 (C terminal). Immunogen maps to a region between residue 1400 and the C-terminus.
    Database link: Q15788

  • Positive control
    • Nuclear extract from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 1 - 4 µg/ml.

Target

  • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • DomainThe C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • Post-translational
    modifications
    Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHe74 antibody
    • Class E basic helix-loop-helix protein 74 antibody
    • F SRC 1 antibody
    • Hin 2 protein antibody
    • Hin2 protein antibody
    • MGC129719 antibody
    • MGC129720 antibody
    • mNRC 1 antibody
    • NCoA 1 antibody
    • NCoA-1 antibody
    • Ncoa1 antibody
    • NCOA1_HUMAN antibody
    • Nuclear receptor coactivator 1 antibody
    • Nuclear receptor coactivator protein 1 antibody
    • NY REN 52 antigen antibody
    • Protein Hin 2 antibody
    • Protein Hin-2 antibody
    • Protein Hin2 antibody
    • Renal carcinoma antigen NY REN 52 antibody
    • Renal carcinoma antigen NY-REN-52 antibody
    • RIP 160 antibody
    • RIP160 antibody
    • SRC 1 antibody
    • SRC-1 antibody
    • Steroid receptor coactivator 1 antibody
    see all

Anti-KAT13A / SRC1 antibody images

  • ab10308 Immunoprecipitate KAT13A / SRC1 in human Huh-7 whole cell lysate. 1,000,000 cells were lysed and incubated with primary antibody at 4µg/mg lysate and Protein A matrix for 20 hours at 4°C. For western blotting an undiluted Abcam`s ab2859, Rabbit polyclonal to KAT13A / SRC1 was used.

    See Abreview

  • ab10308 used in Immunoprecipitation.
    Total protein input is 1 x 106 cells.
    Whole cell lysate was prepared from VERO cells expressing recombinant SRC1 protein.
    Protein A matrix was used for the Immunoprecipitation step.
    ab10308 used a 4µg/mg lysate for 16 hours at 4°C.
    ab2859 was then used for Western Blot at 1µg/mg lysate.

    See Abreview

References for Anti-KAT13A / SRC1 antibody (ab10308)

ab10308 has not yet been referenced specifically in any publications.

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Read More
Application Immunoprecipitation
Sample African Green Monkey Cell lysate - whole cell (Vero cells)
Total protein in input 1e+006 cells
Specification Vero cells
Treatment cells expressing recombitant SRC1 protein
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Aug 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (el4)
Total protein in input 2e+006 cells
Specification el4
Treatment cell expressing mouse myc tagged SRC1
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted May 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (huh-7 cells)
Total protein in input 1e+006 cells
Specification huh-7 cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Sep 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"