Anti-KAT13A / SRC1 antibody - ChIP Grade (ab2859)


  • Product nameAnti-KAT13A / SRC1 antibody - ChIP Grade
    See all KAT13A / SRC1 primary antibodies
  • Description
    Rabbit polyclonal to KAT13A / SRC1 - ChIP Grade
  • SpecificityDetects steroid receptor coactivator-1 (SRC-1).
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, Flow Cyt, ChIP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Bird
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 8-24.


    Database link: Q15788
    (Peptide available as ab5848)

  • Positive control
    • HeLa cell lysate

Associated products


Our Abpromise guarantee covers the use of ab2859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 3-5µg for 106 cells.
ChIP Use at an assay dependent concentration. PubMed: 19005469
ICC/IF 1/200.


  • FunctionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • DomainThe C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • Post-translational
    Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHe74 antibody
    • Class E basic helix-loop-helix protein 74 antibody
    • F SRC 1 antibody
    • Hin 2 protein antibody
    • Hin2 protein antibody
    • MGC129719 antibody
    • MGC129720 antibody
    • mNRC 1 antibody
    • NCoA 1 antibody
    • NCoA-1 antibody
    • Ncoa1 antibody
    • NCOA1_HUMAN antibody
    • Nuclear receptor coactivator 1 antibody
    • Nuclear receptor coactivator protein 1 antibody
    • NY REN 52 antigen antibody
    • Protein Hin 2 antibody
    • Protein Hin-2 antibody
    • Protein Hin2 antibody
    • Renal carcinoma antigen NY REN 52 antibody
    • Renal carcinoma antigen NY-REN-52 antibody
    • RIP 160 antibody
    • RIP160 antibody
    • SRC 1 antibody
    • SRC-1 antibody
    • Steroid receptor coactivator 1 antibody
    see all

Anti-KAT13A / SRC1 antibody - ChIP Grade images

  • Immunolocalization of SRC1 in HeLa cells using ab2859.

  • ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.

  • Flow cytometry analysis of SRC1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature.

References for Anti-KAT13A / SRC1 antibody - ChIP Grade (ab2859)

This product has been referenced in:
  • Hurtado A  et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP ; Human . Read more (PubMed: 19005469) »
  • Yasumoto H  et al. GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. J Cell Sci 120:2532-43 (2007). WB . Read more (PubMed: 17623774) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

I am not sure if you intend to send this email to Abcam however we can offer the following products


ab2859; http://...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (huh-7 cells)
Loading amount 10000 cells
Specification huh-7 cells
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 37°C

Dr. Sungsoo Lee

Verified customer

Submitted May 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Jiqiang Zhang

Verified customer

Submitted Jun 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%

Dr. Jiqiang Zhang

Verified customer

Submitted May 23 2007