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Our Abpromise guarantee covers the use of ab2859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 3-5µg for 106 cells.|
|ChIP||Use at an assay dependent concentration. PubMed: 19005469|
Immunolocalization of SRC1 in HeLa cells using ab2859.
Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.
Flow cytometry analysis of SRC1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"