Overview

  • Product nameAnti-KAT13C / NCOA2 antibody
    See all KAT13C / NCOA2 primary antibodies
  • Description
    Rabbit polyclonal to KAT13C / NCOA2
  • SpecificityWe have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese Hamster
  • Immunogen

    corresponding to KAT13C/ NCOA2 aa 700-750.
    Database link: Q15596

  • Positive control
    • Nuclear extract from HeLa cells. IHC-P: FFPE human testis tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab10491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notesIP: Use at a concentration of 1 - 4 µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
    • Involvement in diseaseNote=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
    • Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 bHLH (basic helix-loop-helix) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.
    • DomainContains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
      The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
      Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHe75 antibody
      • Class E basic helix-loop-helix protein 75 antibody
      • glucocorticoid receptor interacting protein 1 antibody
      • GRIP 1 antibody
      • GRIP1 antibody
      • hTIF2 antibody
      • MED1 antibody
      • MGC138808 antibody
      • NCoA 2 antibody
      • NCoA-2 antibody
      • Ncoa2 antibody
      • NCOA2_HUMAN antibody
      • Nuclear receptor coactivator 2 antibody
      • Oncogene FGR antibody
      • p160 antibody
      • SRC 2 antibody
      • src2 antibody
      • Steroid receptor coactivator 2 antibody
      • TIF 2 antibody
      • TIF2 antibody
      • Transcriptional intermediary factor 2 antibody
      see all

    Anti-KAT13C / NCOA2 antibody images

    • IHC image of KAT13C/NCOA2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10491, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



    • Predicted band size : 175 kDa

      ab10491 detection of human NCOA2 (SRC2) by Western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 10 mg for Co-IP) from HeLa cells. Antibody: ab10491used at 0.33 µg/ml (A) for WB and 20 µg/10 mg lysate (B) for IP.  Detection: Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min. (A) or Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2) (B).  

      ab10491 detection of human NCOA2 (SRC2) by Western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 10 mg for Co-IP) from HeLa cells. Antibody: ab10491used at 0.33 µg/ml (A) f

    References for Anti-KAT13C / NCOA2 antibody (ab10491)

    This product has been referenced in:
    • Lit LC  et al. LATS2 is a modulator of estrogen receptor alpha. Anticancer Res 33:53-63 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23267128) »
    • Amazit L  et al. Ligand-Dependent Degradation of SRC-1 Is Pivotal for Progesterone Receptor Transcriptional Activity. Mol Endocrinol 25:394-408 (2011). WB ; Human . Read more (PubMed: 21273440) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (7% Bis-Tris gels)
    Sample Human Cell lysate - whole cell (primary myometrial cells)
    Specification primary myometrial cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Claire Hudson

    Verified customer

    Submitted Dec 22 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (huh-7)
    Total protein in input 1e+006 cells
    Specification huh-7
    Immuno-precipitation step Protein A
    Username

    Abcam user community

    Verified customer

    Submitted Mar 23 2010

    Application Western blot
    Sample Mouse Tissue lysate - whole (liver)
    Loading amount 15 µg
    Specification liver
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 09 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7 cells)
    Loading amount 25000 cells
    Specification huh-7 cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2008

    The epitope recognized by ab10491 maps to a region between residues 750 and 800 of Swiss-Prot entry Q15596 (http://au.expasy.org/cgi-bin/niceprot.pl?Q15596). The epitope recognized by ab10508 maps to a region within the C-terminal 50 residues of Swi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"