SpecificityWe have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
Involvement in diseaseNote=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family. Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
DomainContains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding. Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ab10508 immunoprecipitation of human NCOA2 (SRC2). Samples: Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: ab10508 used at 20 µg/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2).
References for Anti-KAT13C / NCOA2 antibody (ab10508)
This product has been referenced in:
Zhang Y et al. A systems biology-based classifier for hepatocellular carcinoma diagnosis. PLoS One6:e22426 (2011).
Read more (PubMed: 21829460) »