Purification notesab78899 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography
using non phosphopeptide corresponding to the phosphorylation site.
FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
Involvement in diseaseNote=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family. Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
DomainContains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding. Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Western blot - KAT13C / NCOA2 (phospho S736) antibody (ab78899)
All lanes : Anti-KAT13C / NCOA2 (phospho S736) antibody (ab78899) at 1/500 dilution
Lane 1 : extracts from HeLa cells, treated
with TSA (400 nM, 24 hours) with no immunizing peptide Lane 2 : extracts from HeLa cells, treated
with TSA (400 nM, 24 hours) with immunizing peptide at 10 µg
Immunohistochemical analysis of KAT13C / NCOA2 in paraffin embedded human breast carcinoma tissue, using a 1/50 dilution of ab78899.
Left: untreated sample.
Right: sample treated with immunizing peptide.
References for Anti-KAT13C / NCOA2 (phospho S736) antibody (ab78899)
has not yet been referenced specifically in any publications.
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