Overview

  • Product name
    Anti-KAT13D / CLOCK antibody
    See all KAT13D / CLOCK primary antibodies
  • Description
    Rabbit polyclonal to KAT13D / CLOCK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse KAT13D/ CLOCK aa 394-410.
    Sequence:

    SLPETAADKSQDSGSDN


    (Peptide available as ab5855)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: PC12

Applications

Our Abpromise guarantee covers the use of ab5625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 101.5 kDa).Can be blocked with KAT13D / CLOCK peptide (ab5855).

Target

  • Function
    ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
  • Tissue specificity
    Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications
    Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
  • Cellular localization
    Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • bHLHe8 antibody
    • Circadian locomoter output cycles kaput protein antibody
    • Circadian locomoter output cycles protein kaput antibody
    • Circadian Locomotor Output Cycles Kaput antibody
    • Circadium Locomotor Output Cycles Kaput antibody
    • Class E basic helix-loop-helix protein 8 antibody
    • CLOCK antibody
    • Clock circadian regulator antibody
    • Clock homolog antibody
    • Clock protein antibody
    • CLOCK_HUMAN antibody
    • hCLOCK antibody
    • KIAA0334 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT13D / CLOCK antibody (ab5625)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT13D / CLOCK antibody (ab5625)

    ab5625 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5625 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab5625 has not yet been referenced specifically in any publications.

Publishing research using ab5625? Please let us know so that we can cite the reference in this datasheet.

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