• Product name
    Anti-KAT13D / CLOCK antibody
    See all KAT13D / CLOCK primary antibodies
  • Description
    Rabbit polyclonal to KAT13D / CLOCK
  • Specificity
    ab65033 detects endogenous levels of total KAT13D / CLOCK protein.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human KAT13D/ CLOCK

  • Positive control
    • IHC-P: Human skeletal muscle tissue. WB: HUVEC cell extracts.



Our Abpromise guarantee covers the use of ab65033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).


  • Function
    ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
  • Tissue specificity
    Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
  • Cellular localization
    Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHe8 antibody
    • Circadian locomoter output cycles kaput protein antibody
    • Circadian locomoter output cycles protein kaput antibody
    • Circadian Locomotor Output Cycles Kaput antibody
    • Circadium Locomotor Output Cycles Kaput antibody
    • Class E basic helix-loop-helix protein 8 antibody
    • CLOCK antibody
    • Clock circadian regulator antibody
    • Clock homolog antibody
    • Clock protein antibody
    • CLOCK_HUMAN antibody
    • hCLOCK antibody
    • KIAA0334 antibody
    see all


  • ab65033, at a 1/50 dilution, staining KAT13D / CLOCK in paraffin embedded human skeletal muscle tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
  • All lanes : Anti-KAT13D / CLOCK antibody (ab65033) at 1/500 dilution

    Lane 1 : HUVEC cell extract
    Lane 2 : HUVEC cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 95 kDa
    Observed band size : 95 kDa


This product has been referenced in:
  • Lengyel Z  et al. Circadian clocks and tumor biology: what is to learn from human skin biopsies? Gen Comp Endocrinol 188:67-74 (2013). Read more (PubMed: 23608545) »
  • Nagalla S  et al. Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity. Blood 117:5189-97 (2011). WB ; Human . Read more (PubMed: 21415270) »

See all 2 Publications for this product

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