Overview

  • Product name
    Anti-KAT13D / CLOCK antibody
    See all KAT13D / CLOCK primary antibodies
  • Description
    Rabbit polyclonal to KAT13D / CLOCK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 796-846 of Human KAT13D/ CLOCK (NP_004889.1)

  • Positive control
    • HeLa and 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab93804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 2-5 µg/ml.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
    • Tissue specificity
      Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
    • Post-translational
      modifications
      Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
    • Cellular localization
      Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHe8 antibody
      • Circadian locomoter output cycles kaput protein antibody
      • Circadian locomoter output cycles protein kaput antibody
      • Circadian Locomotor Output Cycles Kaput antibody
      • Circadium Locomotor Output Cycles Kaput antibody
      • Class E basic helix-loop-helix protein 8 antibody
      • CLOCK antibody
      • Clock circadian regulator antibody
      • Clock homolog antibody
      • Clock protein antibody
      • CLOCK_HUMAN antibody
      • hCLOCK antibody
      • KIAA0334 antibody
      see all

    Images

    • All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 0.1 µg/ml

      Lane 1 : HeLa at 50 µg
      Lane 2 : HeLa at 15 µg
      Lane 3 : HeLa at 5 µg
      Lane 4 : 293T at 50 µg

      Developed using the ECL technique

      Predicted band size : 95 kDa


      Exposure time : 3 minutes
    • ab93804 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP.
      Lane 1: IP with an antibody which recognizes an upstream epitope of KAT13D/CLOCK
      Lane 2: ab93804 at 3µg/mg of lysate
      Lane 3: control IgG.
      In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
      Detection: Chemiluminescence with an exposure time of 30 seconds

    References

    ab93804 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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