Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab18381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500. Predicted molecular weight: 94 kDa.
ICC/IF 1/100 - 1/400.

Target

  • Function
    Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
  • Tissue specificity
    Expressed in all tissues tested, with most abundant expression in ovary.
  • Sequence similarities
    Belongs to the GCN5 family.
    Contains 1 bromo domain.
    Contains 1 N-acetyltransferase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110051E14Rik antibody
    • AW212720 antibody
    • EC 2.3.1.48 antibody
    • GCN 5 antibody
    • GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2 antibody
    • Gcn5 antibody
    • GCN5 general control of amino-acid synthesis 5-like 2 (yeast) antibody
    • GCN5L2 antibody
    • general control of amino acid synthesis 5-like 2 antibody
    • General control of amino acid synthesis protein 5-like 2 antibody
    • General control of amino acid synthesis, yeast, homolog-like 2 antibody
    • HGCN5 antibody
    • Histone acetyltransferase GCN5 antibody
    • Histone acetyltransferase KAT2A antibody
    • hsGCN5 antibody
    • K(lysine) acetyltransferase 2A antibody
    • KAT2 A antibody
    • KAT2A antibody
    • KAT2A_HUMAN antibody
    • Lysine acetyltransferase 2A antibody
    • MGC102791 antibody
    • PCAF-b antibody
    • STAF97 antibody
    see all

Images

  • ab18381 at 2ug/ml staining human spleen tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was fixed and blocked and incubated with the antibody for 30 minutes. An HRP conjugated goat antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-KAT2A / GCN5 antibody (ab18381)

    Lane 1 : HUVEC
    Lane 2 : HeLa

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-rabbit IgG secondary

    Predicted band size: 94 kDa

  • ab18381 staining KAT2A / GCN5 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Nuclea stained with DAPI (blue)

References

This product has been referenced in:
  • Yi Q  et al. Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Mol Med Rep 15:2511-2520 (2017). ChIP, WB ; Mouse . Read more (PubMed: 28447752) »
  • Liu Y  et al. Akt kinase targets the association of CBP with histone H3 to regulate the acetylation of lysine K18. FEBS Lett 587:847-53 (2013). Read more (PubMed: 23434580) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Specification
spleen
Antigen retrieval step
Heat mediated
Blocking step
Dako CSA II blocker as blocking agent for 5 minute(s) · Concentration: 100
Username

Abcam user community

Verified customer

Submitted Jan 12 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary muscle cells)
Specification
primary muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Aug 16 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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