Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)

Overview

  • Product nameAnti-KAT2B / PCAF antibody - ChIP Grade
    See all KAT2B / PCAF primary antibodies
  • Description
    Rabbit polyclonal to KAT2B / PCAF - ChIP Grade
  • Tested applicationsIHC-P, ChIP, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    EKFFFSKIKEAGLIDK

    (with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF.

  • Positive control
    • Immunoblotting: Whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF. Immunoprecipitation: RIPA lysate, human acute T cell leukemia Jurkat cells.
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab12188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use at an assay dependent dilution. PubMed: 16829531Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 93 kDa. 1/500 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 93 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.
IP Use 5µg for 107 cells. 5-10µg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 107 human acute T cell leukemia Jurkat cells.
ICC/IF Use at an assay dependent dilution. PubMed: 20410122

Target

  • FunctionFunctions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
  • Tissue specificityUbiquitously expressed but most abundant in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the GCN5 family.
    Contains 1 bromo domain.
    Contains 1 N-acetyltransferase domain.
  • DomainThe bromodomain mediates binding to HIV-1 Tat.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF antibody
    • CREBBP associated factor antibody
    • GCN5 antibody
    • GCN5L antibody
    • GCN5L1 antibody
    • Histone acetylase PCAF antibody
    • Histone acetyltransferase KAT2B antibody
    • Histone acetyltransferase PCAF antibody
    • K(lysine) acetyltransferase 2B antibody
    • KAT2B antibody
    • KAT2B_HUMAN antibody
    • Lysine acetyltransferase 2B antibody
    • P antibody
    • P/CAF antibody
    • p300/CBP associated factor antibody
    • P300/CBP-associated factor antibody
    • Pcaf antibody
    see all

Anti-KAT2B / PCAF antibody - ChIP Grade images

  • ab12188 at 2ug/ml staining human thymus tissue sections by IHC-P. The tissue was formaldehyde fixed and then a heat mediated antigen retrieval step was carried out. The antibody was incubated with the tissue for 30 minutes. An HRP conjugated goat antibody was used as the secondary.

    See Abreview

  • Chromatin was prepared from mouse skin epidermis whole tissue lysate.  Cells were fixed with 1% fromaldehyde for 10 minutes.  TPA treated samples were incubated with rabbit IgG, HDAC7 antibody or ab12188 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR.  Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification.

    See Abreview

References for Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)

This product has been referenced in:
  • Puttagunta R  et al. PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system. Nat Commun 5:3527 (2014). Read more (PubMed: 24686445) »
  • Nye MD  et al. The Transcription Factor GLI1 Interacts with SMAD Proteins to Modulate Transforming Growth Factor ß-Induced Gene Expression in a p300/CREB-binding Protein-associated Factor (PCAF)-dependent Manner. J Biol Chem 289:15495-15506 (2014). WB, ChIP ; Human . Read more (PubMed: 24739390) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (bladder)
Specification bladder
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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Verified customer

Submitted Nov 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (lung epithelium)
Specification lung epithelium
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Semiquantitative PCR
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Submitted Jun 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Tissue lysate - whole (skin epidermis)
Specification skin epidermis
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Semiquantitative PCR
Negative control Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification.
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Submitted Feb 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (L cells)
Specification L cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control none
Negative control none
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Submitted Jul 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (thymus)
Specification thymus
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako CSA II as blocking agent for 5 minute(s) · Concentration: neat
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Submitted Jan 16 2007

Thank you for your enquiry. I checked with our source for this product and the 88 kDa that was listed is wrong. The predicted MW of human PCAF is indeed approx 93 kDa. I have updated our online datasheet to reflect this. The band at 98 kDa that you...

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Thank you for your enquiry. PCAF shows extensive sequence similarity to human and mouse GCN5. Ab12188 has not been tested to see if it reacts with GCN5, but it seems that there is a strong possibility that it will react with GCN5 in addition to PCAF. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"