Overview

  • Product nameAnti-KAT2B / PCAF antibody
    See all KAT2B / PCAF primary antibodies
  • Description
    Rabbit polyclonal to KAT2B / PCAF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human KAT2B/ PCAF.

  • Positive control
    • This antibody gave a positive signal in both Human and Mouse Skeletal Muscle tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab110421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).

Target

  • FunctionFunctions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
  • Tissue specificityUbiquitously expressed but most abundant in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the GCN5 family.
    Contains 1 bromo domain.
    Contains 1 N-acetyltransferase domain.
  • DomainThe bromodomain mediates binding to HIV-1 Tat.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF antibody
    • CREBBP associated factor antibody
    • GCN5 antibody
    • GCN5L antibody
    • GCN5L1 antibody
    • Histone acetylase PCAF antibody
    • Histone acetyltransferase KAT2B antibody
    • Histone acetyltransferase PCAF antibody
    • K(lysine) acetyltransferase 2B antibody
    • KAT2B antibody
    • KAT2B_HUMAN antibody
    • Lysine acetyltransferase 2B antibody
    • P antibody
    • P/CAF antibody
    • p300/CBP associated factor antibody
    • P300/CBP-associated factor antibody
    • Pcaf antibody
    see all

Anti-KAT2B / PCAF antibody images

  • All lanes : Anti-KAT2B / PCAF antibody (ab110421) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 46 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-KAT2B / PCAF antibody (ab110421)

ab110421 has not yet been referenced specifically in any publications.

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