Overview

  • Product name
  • Description
    Rabbit polyclonal to KAT3A / CBP
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 144 - 193 (TPAASQALNP QAQKQVGLAT SSPATSQTGP GICMNANFNQ THPGLLNSNS) of Human KAT3A/ CBP (NP_001073315)

  • Positive control
    • Jurkat cell lysate.

Applications

Our Abpromise guarantee covers the use of ab83857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 265 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
  • Involvement in disease
    Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
  • Sequence similarities
    Contains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Domain
    The KIX domain mediates binding to HIV-1 Tat.
  • Post-translational
    modifications
    Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
  • Cellular localization
    Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBP antibody
    • CBP_HUMAN antibody
    • CREB binding protein antibody
    • CREB-binding protein antibody
    • Crebbp antibody
    • Cyclic AMP responsive enhancer binding protein antibody
    • KAT3A antibody
    • RSTS antibody
    • RTS antibody
    • Rubinstein Taybi syndrome antibody
    see all

Images

  • Anti-KAT3A / CBP antibody (ab83857) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 265 kDa
  • ab83857 at 5 µg/ml staining KAT3A / CBP in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab83857 at 5 µg/ml staining KAT3A / CBP in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab83857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83857.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up