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Immunogen was a synthetic peptide, which represented a portion of human E1A binding protein p300 encoded within exon 16 (LocusLink ID 2033).
Our Abpromise guarantee covers the use of ab10486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 264 kDa.Can be blocked with KAT3B / p300 peptide (ab4914).|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab10486 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"