Overview

  • Product nameAnti-KAT3B / p300 antibody - ChIP Grade
    See all KAT3B / p300 primary antibodies
  • Description
    Rabbit polyclonal to KAT3B / p300 - ChIP Grade
  • Tested applicationsIP, WB, IHC-P, CHIPseqmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human E1A binding protein p300 encoded within exons 15 and 16 (LocusLink ID 2033).

  • Positive control
    • Nuclear or cytosolic (S100) extract from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1-4 µg/mg of lysate.
WB 1/5000 - 1/25000. Can be blocked with KAT3B / p300 peptide (ab4914).
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
CHIPseq Use at an assay dependent concentration.

Target

  • FunctionFunctions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity.
  • Involvement in diseaseNote=Defects in EP300 may play a role in epithelial cancer.
    Note=Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with KAT6A.
    Rubinstein-Taybi syndrome 2 (RSTS2) [MIM:613684]: A disorder characterized by craniofacial abnormalities, postnatal growth deficiency, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. Some individuals with RSTS2 have less severe mental impairment, more severe microcephaly, and a greater degree of changes in facial bone structure than RSTS1 patients. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesContains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • DomainThe CRD1 domain (cell cycle regulatory domain 1) mediates transcriptional repression of a subset of p300 responsive genes; it can be de-repressed by CDKN1A/p21WAF1 at least at some promoters. It conatins sumoylation and acetylation sites and the same lysine residues may be targeted for the respective modifications. It is proposed that deacetylation by SIRT1 allows sumoylation leading to suppressed activity.
  • Post-translational
    modifications
    Acetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020.
    Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
    Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.
    Sumoylated; sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
    Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation.
    Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG.
  • Cellular localizationCytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Co-localizes with ROCK2 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CREBBP/EP300 inhibitory protein 1 antibody
    • Cyclic AMP responsive enhancer binding protein antibody
    • E1A associated protein p300 antibody
    • E1A binding protein p300 antibody
    • E1A-associated protein p300 antibody
    • EC 2.3.1.48 antibody
    • EP300 antibody
    • EP300: E1A binding protein p300 antibody
    • EP300_HUMAN antibody
    • Histone acetyltransferase p300 antibody
    • KAT3B antibody
    • p300 HAT antibody
    • RB and P300 binding protein EID 1 antibody
    • Retinoblastoma protein associated protein antibody
    • RSTS2 antibody
    see all

Anti-KAT3B / p300 antibody - ChIP Grade images

  • All lanes : Anti-KAT3B / p300 antibody - ChIP Grade (ab10485) at 1/5000 dilution

    Lane 1 : Human MCF-7 Cell Lysate
    Lane 2 : MDA-MB-231 Cell Lysate

    Lysates/proteins at 50000 cells per lane.

    Secondary
    Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866)
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Solution and Diluent 5% milk in TBS

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KAT3B / p300 with ab10485 at 1/1000 (1µg/ml). Detection: DAB.
  • ab10485 detecting p300 in HeLa cells by Immunoprecipitation .

    Left-hand panel represents a coomassie-blue stained gel loaded with 10mg of HeLa cytosolic (S100) and nuclear (NE) extracts;

    For the Immunoprecipitation experiment (right-hand panel), ab10485 was used at a concentration of 20 µg/10 mg of HeLa whole cell lysate (lanes correspond to 5, 15 and 50 µg of lysate). The flow-through was analysed by WB using ab10485 at a concentration of 0.04 µg/ml.

    The identity of the band marked as "p300" was also confirmed using mass spectrometry.

References for Anti-KAT3B / p300 antibody - ChIP Grade (ab10485)

This product has been referenced in:
  • Muench P  et al. Cutaneous papillomavirus E6 proteins must interact with p300 and block p53-mediated apoptosis for cellular immortalization and tumorigenesis. Cancer Res 70:6913-24 (2010). WB ; Human . Read more (PubMed: 20663910) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (8% gel)
Sample Human Cell lysate - whole cell (MCF-7)
Specification MCF-7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Nov 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"