Anti-KAT4 / TBP Associated Factor 1 antibody (ab28450)

Overview

  • Product name
    Anti-KAT4 / TBP Associated Factor 1 antibody
    See all KAT4 / TBP Associated Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to KAT4 / TBP Associated Factor 1
  • Specificity
    ab28450 recognises transcription initiation factor TFIID subunit 1 (TAF1).
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide derived from residues 1823 - 1872 (C-terminal) of human TAF1.

  • Positive control
    • Daudi cell lysate, Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab28450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 212 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use 5-10 µg for 25 µg of chromatin.

Target

  • Function
    Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
  • Involvement in disease
    Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
  • Sequence similarities
    Belongs to the TAF1 family.
    Contains 2 bromo domains.
    Contains 1 HMG box DNA-binding domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Phosphorylated by casein kinase II in vitro.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BA2R antibody
    • CCG1 antibody
    • CCGS antibody
    • Cell cycle G1 phase defect antibody
    • Cell cycle gene 1 protein antibody
    • Complementation of cell cycle block G1 to S antibody
    • DYT3 antibody
    • N TAF1 antibody
    • NSCL2 antibody
    • OF antibody
    • p250 antibody
    • TAF 1 antibody
    • TAF(II)250 antibody
    • TAF1 antibody
    • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
    • TAF1_HUMAN antibody
    • TAF2A antibody
    • TAFII-250 antibody
    • TAFII250 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
    • TBP associated factor 250 kDa antibody
    • TBP-associated factor 250 kDa antibody
    • Transcription factor TFIID p250 polypeptide antibody
    • Transcription initiation factor TFIID 250 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 1 antibody
    • XDP antibody
    see all

Anti-KAT4 / TBP Associated Factor 1 antibody images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • This image shows human kidney stained with ab28450 at 8µg/ml. Magnification 400x.
  • Anti-KAT4 / TBP Associated Factor 1 antibody (ab28450) at 0.5 µg/ml + Daudi cell lysate

    Predicted band size : 212 kDa
  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28450 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
       

References for Anti-KAT4 / TBP Associated Factor 1 antibody (ab28450)

This product has been referenced in:
  • Iwata J  et al. Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells. J Biol Chem 285:4975-82 (2010). WB ; Mouse . Read more (PubMed: 19959467) »
  • van Essen D  et al. Two modes of transcriptional activation at native promoters by NF-kappaB p65. PLoS Biol 7:e73 (2009). ChIP ; Mouse . Read more (PubMed: 19338389) »

See all 2 Publications for this product

Product Wall

Application
ChIP
Sample
Mouse Cell lysate - nuclear (ESCs)
Specification
ESCs
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1 % (37ºC)
Positive control
samples
Username

Abcam user community

Verified customer

Submitted Sep 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human fibromatosis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - Tween-20
Specification
Human fibromatosis
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample
Dog Tissue sections (MDCK pellets in paraffin)
Specification
MDCK pellets in paraffin
Permeabilization
Yes - Tween-20
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 04 2015

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody has been applied by Abcams chromatin postdoc scientist by adding 5-10µg of antibody to 25µg of chromatin. Please can you tell me ...

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