Anti-KAT4 / TBP Associated Factor 1 antibody (ab75656)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab75656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 213 kDa (predicted molecular weight: 213 kDa).Can be blocked with Human KAT4 / TBP Associated Factor 1 peptide (ab87365).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionLargest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
  • Involvement in diseaseDefects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
  • Sequence similaritiesBelongs to the TAF1 family.
    Contains 2 bromo domains.
    Contains 1 HMG box DNA-binding domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Phosphorylated by casein kinase II in vitro.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BA2R antibody
    • CCG 1 antibody
    • CCG1 antibody
    • CCGS antibody
    • Cell cycle G1 phase defect antibody
    • Cell cycle gene 1 protein antibody
    • Complementation of cell cycle block G1 to S antibody
    • DYT3 antibody
    • DYT3 protein antibody
    • N TAF1 antibody
    • NSCL 2 antibody
    • NSCL2 antibody
    • OF antibody
    • P250 antibody
    • TAF 1 antibody
    • TAF 2A antibody
    • TAF(II)250 antibody
    • TAF1 antibody
    • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
    • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor antibody
    • TAF1 RNA polymerase II TATA box binding protein associated factor 250kDa antibody
    • TAF1_HUMAN antibody
    • TAF2A antibody
    • TAFII 250 antibody
    • TAFII-250 antibody
    • TAFII250 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II A antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
    • TBP associated factor 250 kDa antibody
    • TBP-associated factor 250 kDa antibody
    • Transcription factor TFIID p250 polypeptide antibody
    • Transcription initiation factor TFIID 250 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 1 antibody
    • XDP antibody
    see all

Anti-KAT4 / TBP Associated Factor 1 antibody images

  • All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody (ab75656) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 213 kDa
    Observed band size : 213 kDa
  • ICC/IF image of ab75656 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75656, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of KAT4/TBP Associated Factor 1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75656, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-KAT4 / TBP Associated Factor 1 antibody (ab75656)

ab75656 has not yet been referenced specifically in any publications.

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