Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab85533)

Overview

  • Product nameAnti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade
    See all KAT4 / TBP Associated Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to KAT4 / TBP Associated Factor 1 - ChIP Grade
  • Tested applicationsChIP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 1800-1849 (MMSYEGDGGE ASHGLEDSNI SYGSYEEPDP KSNTQDTSFS SIGGYEVSEE) of Human KAT4/ TBP Associated Factor 1, NP_004597.

  • Positive control
    • Human fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40, 213 kDa (predicted molecular weight: 213 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionLargest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
  • Involvement in diseaseDefects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
  • Sequence similaritiesBelongs to the TAF1 family.
    Contains 2 bromo domains.
    Contains 1 HMG box DNA-binding domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Phosphorylated by casein kinase II in vitro.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BA2R antibody
    • CCG1 antibody
    • CCGS antibody
    • Cell cycle G1 phase defect antibody
    • Cell cycle gene 1 protein antibody
    • Complementation of cell cycle block G1 to S antibody
    • DYT3 antibody
    • N TAF1 antibody
    • NSCL2 antibody
    • OF antibody
    • P250 antibody
    • TAF 1 antibody
    • TAF(II)250 antibody
    • TAF1 antibody
    • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
    • TAF1_HUMAN antibody
    • TAF2A antibody
    • TAFII-250 antibody
    • TAFII250 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
    • TBP associated factor 250 kDa antibody
    • TBP-associated factor 250 kDa antibody
    • Transcription factor TFIID p250 polypeptide antibody
    • Transcription initiation factor TFIID 250 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 1 antibody
    • XDP antibody
    see all

Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab85533) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 213 kDa
    Observed band size : 213 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 6-18%

References for Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab85533)

ab85533 has not yet been referenced specifically in any publications.

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