Human Predicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
Synthetic peptide corresponding to a region within the internal sequence amino acids 1800-1849 (MMSYEGDGGE ASHGLEDSNI SYGSYEEPDP KSNTQDTSFS SIGGYEVSEE) of Human KAT4/ TBP Associated Factor 1, NP_004597.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Detects a band of approximately 40, 213 kDa (predicted molecular weight: 213 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionLargest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
Involvement in diseaseDefects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
Sequence similaritiesBelongs to the TAF1 family. Contains 2 bromo domains. Contains 1 HMG box DNA-binding domain. Contains 2 protein kinase domains.
Post-translational modificationsPhosphorylated by casein kinase II in vitro.
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Western blot - KAT4 / TBP Associated Factor 1 antibody (ab85533)
Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab85533) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 213 kDa Observed band size : 213 kDa Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.Gel concentration 6-18%