Overview

  • Product nameAnti-KAT5 / Tip60 antibody
    See all KAT5 / Tip60 primary antibodies
  • Description
    Rabbit polyclonal to KAT5 / Tip60
  • Specificityab23886 gives nuclear staining for Tip60 in interphase cells (see below), which is lost upon entry into mitosis (not shown). This signal can be successfully quenched using the immunizing peptide ab26349.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Tip60.

    (Peptide available as ab26349.)

  • Positive control
    • This antibody gave a positive signal in MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab23886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
  • Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
  • Post-translational
    modifications
    Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
    Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
    Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
  • Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa Tat interactive protein antibody
    • 60 kDa Tat-interactive protein antibody
    • cPLA(2) interacting protein antibody
    • cPLA(2)-interacting protein antibody
    • cPLA2 antibody
    • cPLA2 interacting protein antibody
    • ESA1 antibody
    • Histone acetyltransferase HTATIP antibody
    • Histone acetyltransferase KAT5 antibody
    • HIV 1 Tat interactive protein antibody
    • HIV 1 Tat interactive protein, 60kDa antibody
    • HIV-1 Tat interactive protein antibody
    • HTATIP antibody
    • HTATIP1 antibody
    • K(lysine) acetyltransferase 5 antibody
    • K-acetyltransferase 5 antibody
    • KAT5 antibody
    • KAT5_HUMAN antibody
    • Lysine acetyltransferase 5 antibody
    • PLIP antibody
    • Tat interacting protein, 60kDa antibody
    • TIP antibody
    • Tip60 antibody
    see all

Anti-KAT5 / Tip60 antibody images

  • ab23886 staining KAT5 / Tip60 in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • HeLa cells were plated on poly-D lysine coated cover slips, fixed with 4% w/v paraformaldehyde (10 min), permeabilised in 0.1% Triton X-100 in PBS (10 min), incubated for 1 hour at 37°C with ab23886 and stained with a FITC-conjugated secondary antibody and DAPI. Left panel: FITC signal. Right panel: FITC+DAPI.

    ab23886 gives a specific nuclear staining for Tip60. Nucleoli are stained more intensely, as also demonstrated by co-staining with known nucleolar makers (not shown). The signal is blocked by pre-incubation of ab23886 with the cognate immunogenic peptide.
  • ICC/IF image of ab23886 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23886, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-KAT5 / Tip60 antibody (ab23886)

This product has been referenced in:
  • Yasunaga A  et al. Genome-wide RNAi screen identifies broadly-acting host factors that inhibit arbovirus infection. PLoS Pathog 10:e1003914 (2014). ICC/IF . Read more (PubMed: 24550726) »
  • Xu S  et al. Epigenetic control of learning and memory in Drosophila by Tip60 HAT action. Genetics 198:1571-86 (2014). Read more (PubMed: 25326235) »

See all 6 Publications for this product

Product Wall

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Expiration date:06. September 2012

I am very pleased to hear your customer would like to accept our offer and test ab23886 in IP.

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Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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Submitted Mar 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 50 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Submitted Jan 24 2012

Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (3rd intar larvae)
Loading amount 30 µg
Specification 3rd intar larvae
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Nov 22 2011

I am very pleased to hear you would like to accept our offer and test ab23886 in drosophila. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for drosophila and include this cod...

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Thank you very much for your interest in ab23886. I think it is a good idea to test this antibody in western blotting as it is already a tested application. Please see below for an ongoing testing discount promotion you may be interested in: To our kno...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 20 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HeLa #Mef #F9 #PC12)
Loading amount 30 µg
Specification HeLa #Mef #F9 #PC12
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Aug 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"