The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
Sequence similaritiesBelongs to the MYST (SAS/MOZ) family. Contains 1 C2HC-type zinc finger.
Post-translational modificationsSumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies. Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity. Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
ab75617, at a 1/10 dilution, staining KAT5 / Tip60 in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab75617 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
References for Anti-KAT5 / Tip60 antibody (ab75617)
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