Anti-KAT5 / Tip60 (phospho S90) antibody (ab111588)

Overview

  • Product nameAnti-KAT5 / Tip60 (phospho S90) antibody
    See all KAT5 / Tip60 primary antibodies
  • Description
    Rabbit polyclonal to KAT5 / Tip60 (phospho S90)
  • Specificityab111588 detects endogenous levels of KAT5 / Tip60 only when phosphorylated at serine 90.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from Human KAT5/ Tip60 around the phosphorylation site of Ser90.

  • Positive control
    • Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab111588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
  • Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
  • Post-translational
    modifications
    Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
    Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
    Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
  • Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa Tat interactive protein antibody
    • 60 kDa Tat-interactive protein antibody
    • cPLA(2) interacting protein antibody
    • cPLA(2)-interacting protein antibody
    • cPLA2 antibody
    • cPLA2 interacting protein antibody
    • ESA1 antibody
    • Histone acetyltransferase HTATIP antibody
    • Histone acetyltransferase KAT5 antibody
    • HIV 1 Tat interactive protein antibody
    • HIV 1 Tat interactive protein, 60kDa antibody
    • HIV-1 Tat interactive protein antibody
    • HTATIP antibody
    • HTATIP1 antibody
    • K(lysine) acetyltransferase 5 antibody
    • K-acetyltransferase 5 antibody
    • KAT5 antibody
    • KAT5_HUMAN antibody
    • Lysine acetyltransferase 5 antibody
    • PLIP antibody
    • Tat interacting protein, 60kDa antibody
    • TIP antibody
    • Tip60 antibody
    see all

Anti-KAT5 / Tip60 (phospho S90) antibody images

  • ab111588, at a 1/50 dilution, staining KAT5 / Tip60 in paraffin embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-KAT5 / Tip60 (phospho S90) antibody (ab111588)

ab111588 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (GTL-16)
Specification GTL-16
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jan 06 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"