Our Abpromise guarantee covers the use of ab70183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).|
|ICC/IF||1/100 - 1/500.|
|IP||Use a concentration of 5 µg/ml.|