Recombinant
RabMAb

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)

Overview

  • Product name
    Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)]
    See all KAT7 / Hbo1 / MYST2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human KAT7/ Hbo1/ MYST2 aa 50-150.
    Database link: O95251

  • Positive control
    • HeLa, Jurkat, MCF7, and HepG2 cell lysates; Permeabilized HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.
    • Tissue specificity
      Ubiquitously expressed, with highest levels in testis.
    • Sequence similarities
      Belongs to the MYST (SAS/MOZ) family.
      Contains 1 C2HC-type zinc finger.
    • Domain
      The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
      The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hbo 1 antibody
      • HBO1 antibody
      • HBOa antibody
      • Histone acetyltransferase binding to hORC1 antibody
      • Histone acetyltransferase binding to ORC1 antibody
      • Histone acetyltransferase KAT7 antibody
      • Histone acetyltransferase MYST2 antibody
      • K(lysine) acetyltransferase 7 antibody
      • KAT 7 antibody
      • KAT7 antibody
      • Lysine acetyltransferase 7 antibody
      • MOZ antibody
      • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
      • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
      • MYST 2 antibody
      • MYST histone acetyltransferase 2 antibody
      • MYST protein 2 antibody
      • MYST-2 antibody
      • MYST2 antibody
      • MYST2_HUMAN antibody
      • SAS 2 antibody
      • SAS2 and TIP60 protein 2 antibody
      • TIP60 protein 2 antibody
      • YBF2/SAS3 antibody
      • ZC2HC7 antibody
      see all

    Images

    • All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 71 kDa
    • ab124993 at 1/100 dilution staining KAT7 / Hbo1 / MYST2 in permeabilized HeLa cells by Flow cytometry (red) or negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . Read more (PubMed: 24726359) »

    See 1 Publication for this product

    Customer reviews and Q&As

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    The overall homology between Human and Xenopus is 93%. Thus, in general there is good chance for an antibody raised against human to work in Xenopus.
    For ab31506 the homology of the immunogen with Xenopus is 100...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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