Anti-KAT8 / MYST1 / MOF antibody (ab72056)

Overview

  • Product nameAnti-KAT8 / MYST1 / MOF antibody
    See all KAT8 / MYST1 / MOF primary antibodies
  • Description
    Rabbit polyclonal to KAT8 / MYST1 / MOF
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human KAT8/ MYST1/ MOF (BAB13924.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionHistone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM.
  • Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
    Contains 1 chromo domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone acetyltransferase KAT8 antibody
    • Histone acetyltransferase MYST1 antibody
    • hMOF antibody
    • K(lysine) acetyltransferase 8 antibody
    • KAT 8 antibody
    • Lysine acetyltransferase 8 antibody
    • MOF antibody
    • MOZ antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 antibody
    • MYST 1 antibody
    • MYST histone acetyltransferase 1 antibody
    • myst protein 1 antibody
    • MYST-1 antibody
    • MYST1 antibody
    • MYST1_HUMAN antibody
    • Ortholog of Drosophila males absent on the first (MOF) antibody
    • Probable histone acetyltransferase MYST1 antibody
    • SAS2 and TIP60 protein 1 antibody
    • SAS2 antibody
    • SAS3 antibody
    • TIP60 protein 1 antibody
    • YBF2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC8 antibody
    see all

Anti-KAT8 / MYST1 / MOF antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KAT8 / MYST1 / MOF with ab72056 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-KAT8 / MYST1 / MOF antibody (ab72056) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,19 kDa,28 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human KAT8 / MYST1 / MOF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72056 at 3 µg/mg lysate. Subsequent WB detection was done using ab72056 at 1 µg/ml.
  • IHC image of ab72056 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72056, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-KAT8 / MYST1 / MOF antibody (ab72056)

ab72056 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The epitope recognized by ab72056 is 86% identical between human and mouse. There are 2 mismatches out of 15 near the N-terminus. Although it can be hard to predict cross-species reactivity when there is not 100% ide...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"