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Western blot - katanin p80 antibody (ab37555)
(enlarge)
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Product Name
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katanin p80 antibody
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See all katanin p80 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to katanin p80
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Immunogen
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Synthetic peptide, corresponding to N terminal amino acids 1-220 of Human katanin p80 subunit B1.
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Reacts with
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Hu, Ms, Rat
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000. Detects a band of approximately 80 kDa for the fusion protein (predicted molecular weight for the native protein: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Predominantly cytoplasmic. Also localized to the interphase centrosome and mitotic spindle poles.
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, whereas the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this katanin p80 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for katanin p80 antibody images, references, products related to ab37555 and other tools.
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katanin p80 antibody images:
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 Western blot - katanin p80 antibody (ab37555)
katanin p80 antibody (ab37555) at 1/2000 dilution + E. coli derived fusion protein at 0.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
A fusion protein was used as positive control; hence the difference in band size compared with that expected.
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Search PubMed (MEDLINE) for references to katanin p80
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katanin p80 antibody - more information
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katanin p80 antibody - related products:
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