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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
katanin p80 protein (T7 Tag)
Protein description
Recombinant full length protein (Human) .This protein has a T7 tag at the N-terminus.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Sodium Azide
Constituents: 10mM Tris, 0.1% Triton X-100, 10mM DTT, pH 8.0
Concentration
Concentration information loading...
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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SDS-PAGE - katanin p80 peptide (ab40498)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution.
Mass Spectrometry: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.
Sequence similarities
Belongs to the WD repeat KATNB1 family.
Contains 6 WD repeats.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton. Predominantly cytoplasmic. Localized to the interphase centrosome and mitotic spindle poles.
Target information above from: UniProt accessionQ9BVA0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
katanin p80 protein (T7 Tag) images:
SDS-PAGE - katanin p80 peptide (ab40498)
Analysis of ab40498, using 1µg of katanin p80 recombinant protein. 4-20% SDS gradient gel. Coomassie blue staining.
References for katanin p80 protein (T7 Tag) (ab40498)
ab40498 has not yet been referenced specifically in any publications.
Publishing research using ab40498? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"