Overview

  • Product nameAnti-KATNA1 antibody
    See all KATNA1 primary antibodies
  • Description
    Mouse polyclonal to KATNA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length KATNA1 protein (Human)

  • Positive control
    • KATNA1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionSevers microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth.
  • Sequence similaritiesBelongs to the AAA ATPase family.
  • DomainThe N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain and ATP, which promotes oligomerization.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Predominantly cytoplasmic. Also localized to the interphase centrosome. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Katanin p60 ATPase containing subunit A1 antibody
    • Katanin p60 ATPase-containing subunit A1 antibody
    • Katanin p60 subunit A1 antibody
    • KATNA1 antibody
    • KTNA1_HUMAN antibody
    • OTTHUMP00000017392 antibody
    • OTTHUMP00000042860 antibody
    • p60 katanin antibody
    see all

Anti-KATNA1 antibody images

  • All lanes : Anti-KATNA1 antibody (ab67206)

    Lane 1 : KATNA1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate


    Predicted band size : 56 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


  • Predicted band size : 56 kDa
    Observed band size : 56 kDa

References for Anti-KATNA1 antibody (ab67206)

This product has been referenced in:
  • Butler R  et al. Genetic and chemical modulation of spastin-dependent axon outgrowth in zebrafish embryos indicates a role for impaired microtubule dynamics in hereditary spastic paraplegia. Dis Model Mech 3:743-51 (2010). WB ; Zebrafish . Read more (PubMed: 20829563) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. Endogenous, un-transfected lysate is used as control sample for comparing generated antibody's reactivity against the target protein on western blot. It is difficult to know the exact quantity of protein expressed by the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"